What is CreERT2?

CreERT2 encodes a Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2) that requires the presence of tamoxifen for activity. No functional Cre recombinase activity was found in CreERT2 mice crossed with reporter mice in the absence of tamoxifen.

What is Cre expression?

Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme (38kDa) is a member of the integrase family of site specific recombinase and it is known to catalyse the site specific recombination event between two DNA recognition sites (LoxP sites).

What are Cre cells?

The Cre protein is a site-specific DNA recombinase that can catalyse the recombination of DNA between specific sites in a DNA molecule. These sites, known as loxP sequences, contain specific binding sites for Cre that surround a directional core sequence where recombination can occur.

How does Cre lox recombination work?

How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event between two loxP sites, which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized and bound by a Cre protein, forming a dimer.

What is Rosa26 locus?

The Rosa26 locus in the mouse genome is a “safe harbor” which allows researchers to express genes of interest. Different gene targeting technologies (embryonic stem cells; CRISPR) are used to make specific DNA insertions at the Rosa26 locus.

What is UBC Cre?

Also Known As:UBC-Cre-ERT2 When these UBC-Cre-ERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in widespread cells/tissues.

What is Cre LOX used for?

The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. It gives mouse researchers sophisticated control over the location and timing of gene expression. Cre/lox is usually used to make knockout alleles, but it can also be used to activate gene expression.

Is Cre lox reversible?

All recombination events mediated by FLP or Cre are reversible. The loxP and FRT target sites have been engineered to avoid this re-inversion issue.

How contagious is Cre?

How are CRE germs spread? CRE are usually spread person to person through contact with infected or colonized people, particularly contact with wounds or stool (poop). This contact can occur via the hands of healthcare workers, or through medical equipment and devices that have not been correctly cleaned.

Is Rosa26 a gene?

Rosa26 Knockin Known in scientific circles as Gt(ROSA)26Sor, the Rosa26 locus is a specific genetic site that is located on mouse chromosome 6, and is considered to be one of the ideal locations where knock-ins of interest can be targeted.

What is safe harbor locus?

Safe harbor sites (SHS) are genomic loci where genes or other genetic elements can be safely inserted and expressed. These SHS are critical for effective human disease gene therapies; for investigating gene structure, function and regulation; and for cell marking and tracking.

What is CMV cre?

Allele Symbol: Tg(CMV-cre)1Cgn Homozygous transgenic mice are viable and fertile. This transgene expresses Cre recombinase under the control of a human cytomegalovirus (CMV) promoter, active in most cells and tissues.

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Which is better CRI 5 or CRE 5?

CR 5, CRI 5, CRN 5, CRE 5 GRUNDFOSDATA BOOKLET CR 5, CRI 5, CRN 5, CRE 5, CRIE 5, CRNE 5 Vertical multistage centrifugal pumps 50 Hz Lenntech [email protected] www.lenntech.com Performance curves/technical data 38 6 CR, CRE 5 TM02 7294 3605 0 1 2 3 4 5 6 7 8Q [m³/h] 0 20 40 60 80 100 120 140 160 180 200 220 240 260 H [m]

Which is the most common type of CRE?

Types of CRE are sometimes known as KPC (Klebsiella pneumoniae carbapenemase) and NDM (New Delhi Metallo-beta-lactamase). KPC and NDM are enzymes that break down carbapenems and make them ineffective.

Which is the best CRE program for real estate?

Includes profiles of the top graduate real estate programs as well as commentaries on the subject. A.CRE Accelerator. Discover the CRE modeling fundamentals you didn’t learn in school, with our 16-course, case-based real estate financial modeling Accelerator. A.CRE Careers. Learn about and prepare for a career in commercial real estate.

How is cadherin 5 promoter used in Cre recombinase?

The cadherin 5 promoter was used to drive expression of cre recombinase in the adult epithelium. Three lines were created. Alva JA; Zovein AC; Monvoisin A; Murphy T; Salazar A; Harvey NL; Carmeliet P; Iruela-Arispe ML. 2006. VE-Cadherin-Cre-recombinase transgenic mouse: A tool for lineage analysis and gene deletion in endothelial cells.