What is FuGENE transfection reagents and how does it work?

FuGENE 6 Transfection Reagent is a nonliposomal, multicomponent reagent proven to efficiently transfect more than 700 cell types. It can be used in the presence or absence of serum, and its minimal cytotoxicity eliminates the need to change medium after transfection.

What is the transfection reagent?

Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. However, any transfection reagent will require optimization for each cell type and conditions (e.g., confluency, passage number) in which it is used to ensure efficient gene transfer and expression.

How does the FuGene transfection reagent ( a ) work?

FuGENE®6 Transfection Reagent(a)is a nonliposomal reagent that transfects DNA into a wide variety of cell lines with high effi ciency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.

How big is a 1mL FuGene 6 reagent?

The 1ml size contains suffi cient reagent to transfect 333µg of DNA. This is equivalent to over 3,300 wells in 96-well plates at a 3:1 FuGENE® 6 Transfection Reagent:DNA ratio (0.3µl reagent:100ng DNA per well). The actual number of transfections will vary with reagent:DNA ratio, transfection volume and cell type.

What are the transfection conditions for FuGene HD?

Use the FuGENE® HD protocol database to see if transfection conditions for your cell line are available. The database includes over 25 cancer models and provides transfection protocols specific for each cell line. High expression levels and the low toxicity of FuGENE® HD can bring better performance for imaging experiments.

What is the ratio of FuGene HD to DNA?

For successful transfection of DNA into cultured cells, the ratio of FuGENE® HD Transfection Reagent:DNA must be. optimized. Ratios of 1.5:1 to 4:1 FuGENE® HD Transfection Reagent:DNA work well with many cell lines, but ratios. outside of this range (4.5:1 to 6:1) may be optimal for other cell types or applications.