Users' questions

How do you choose a solvent for column chromatography?

How do you choose a solvent for column chromatography?

Other Factors to Consider When Choosing a Solvent System There are two other factors to consider: price and boiling point. If two solvent systems give similarly good separation, the cheapest of the non-halogenated solvents should be used. Typically, this will result in a hexanes:ethyl acetate mixture.

How do you determine the best solvent for TLC?

For instance, in determining the solvent system for a flash chromatography procedure, the ideal system is the one that moves the desired component of the mixture to a TLC Rf of 0.25-0.35 and will separate this component from its nearest neighbor by difference in TLC Rf values of at least 0.20.

How do I choose a solvent for HPLC?

Critical considerations in HPLC solvent selection

  1. Cost. Cost is an important consideration as HPLC requires superior purity grade solvents and it is common to see dozens of HPLC systems operating round the clock in large laboratories.
  2. Solubility.
  3. Absorbance.
  4. Volatility.
  5. Viscosity.
  6. Inertness.
  7. Water.

What is solvent system in TLC?

In reversed-phase chromatography, the typical solvent systems are: Mixtures of water or aqueous buffers and water miscible organic solvents such as acetonitrile (ACN), methanol, and tetrahydrofuran (THF). Other solvents can be used such as ethanol (EtOH) & isopropanol (IPA).

How to choose the best solvent for column chromatography?

Choosing a Solvent System. Common solvents and solvent systems for column and flash chromatography. To get a good separation between your product and any impurities and, where possible, to use as little solvent as possible, the key is to test potential solvent systems before setting up your column.

How to prepare a flash column for chromatography?

Preparing and Running a Flash Column: 1) Determine the dry, solvent-free weight of the mixture that you want to separate. 2) Determine the solvent system for the column by using TLC (see TLC Handout). You should aim for Rf values between 0.2 and 0.3. If your mixture is complicated then this may not be possible.

What does eluent mean in flash column chromatography?

This simply means that you increase the polarity of the solvent running through the column (eluent) throughout the course of the purification. This technique will be described in more detail later in the handout, but for the TLC analysis you should determine which solvent systems put the different spots in the 0.2 to 0.3 Rf range.

What kind of Pipet to use for column chromatography?

If your compound is a non-viscous oil, it is easiest to apply it neat. You can use a long Pasteur pipet to apply the liquid to the column and then rinse any traces of it into the column using the predetermined solvent system. In Solution: