Where is the wobble position on tRNA?

The anticodon loops of almost all tRNAs contain several modified nucleotides. Among these, the most important are nucleotides in the positions 34 and 37. The nucleotide in position 34 (so-called ‘wobble’ position) pairs with the third mRNA codon base in the aminoacyl-tRNA binding site (A-site) during decoding4,8.

What is the wobble effect in tRNA?

Some tRNAs bind to multiple codons (“wobble”) Some tRNAs can form base pairs with more than one codon. Wobble pairing lets the same tRNA recognize multiple codons for the amino acid it carries. For example, the tRNA for phenylalanine has an anticodon of 3′-AAG-5′.

What is the wobble in codon anticodon recognition?

Often one tRNA can recognize more than one codon. The rules governing the recognition patterns are summarized in the wobble hypothesis, which states that the pairing between codon and anticodon at the first two codon positions always follows the usual rules, but that exceptional “wobbles” occur at the third position.

Where does wobble occur in tRNA anticodon pairing?

“Wobble”. Pairing of the tRNA anticodon with the mRNA codon proceeds from the 5′ end of the codon. Once the first two positions are paired, exact base pairing of the third position is less critical.

What are the wobble positions of a codon?

Codon wobble positions tRNA 5′ anticodon base mRNA 3′ codon base G U,C C G k 2 C A A U,C, (A),G

What does wobble mean in relation to tRNA?

According to this hypothesis, only the first two bases of the codon have a precise pairing with the bases of the anticodon of tRNA, while the pairing between the third bases of codon and anticodon may Wobble (wobble means to sway or move unsteadily). The phenomenon permits a single tRNA to recognize more than one codon.

How many tRNA genes are needed for wobble position?

Unlike the standard base pairing rules, the base pairing rules for this “wobble position are shown below: There are 86 tRNA genes on the E. coli chromosome, but only 47 different tRNAs are required to recognize all of the possible sense codons.