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Which restriction enzymes are compatible ends?

Which restriction enzymes are compatible ends?

Compatible Cohesive Ends and Generation of New Restriction Sites

Enzyme Ligated To Recleaved By
MluI (A/CGCGT) AscI, BssHII BstUI, HhaI
MseI (T/TAA) AseI MseI
BfaI, Csp6I, NdeI
NarI (GG/CGCC) AccI (GT/CGAC), AclI, ClaI, BstBI, TaqI-v2

Which type of restriction enzymes does EcoRI belong to?

EcoRI (pronounced “eco R one”) is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites, and is also a part of the restriction modification system.

Which restriction enzymes produce compatible cohesive ends?

Compatible Cohesive Ends and Generation of New Restriction Sites

Enzyme Ligated To Recleaved By
(GR/CGYC) AccI (GT/CGAC), ClaI, BstBI, TaqI-v2
(GA/CGYC) AciI, HinP1I HgaI

What does EcoRI bind to?

EcoRI is a type II restriction endonuclease with a palindromic target site on DNA—GAATTC—that it binds to as a dimer. It cleaves foreign DNA in an E. coli host in the presence of a Mg2+ ion cofactor.

How do I inactivate the enzyme?

Boiling water is sure to inactivate most enzymes. 10 min in boiling water would be enough to inactivate most enzymes. After inactivation you can start you regular assay and measure activity.

Where does EcoRI cut?

EcoRI makes one cut between the G and A in each of the DNA strands (see below). After the cuts are made, the DNA is held together only by the hydrogen bonds between the four bases in the middle. Hydrogen bonds are weak, and the DNA comes apart.

How do restriction enzymes create recombinant DNA?

Restriction enzymes cut DNA by breaking internal phosphodiester bonds thus forming a nick to introduce desirable gene or DNA fragment. Then ligase is used to seal and join the DNA molecules together. This forms the recombinant DNA. Thus restriction enzymes and ligase are the enzymes necessary to make recombinant DNA.

What is the biological function of a restriction enzyme?

Restriction enzymes are found in many different strains of bacteria, where their biological role is to participate in cell defense. These enzymes “restrict” foreign DNA (e.g. viral) that enter the cell, by destroying it.