Can Qubit measure RNA?

Qubit™ RNA HS Assay Kit. New—The Qubit™ 4 Fluorometer can now measure RNA quality in addition to providing accurate quantitation of dsDNA, ssDNA, RNA & protein.

How does Qubit RNA work?

The Qubit fluorometer uses fluorescent dyes that emit signals only when bound to the specific target molecules (DNA or RNA) even in the presence of free nucleotides, degraded nucleic acids, or protein contaminants.

How much does a Qubit fluorometer cost?

Qubit 4 Fluorometer

What does the Qubit measure?

The Qubit fluorometer is a DNA quantification device based on the fluorescence intensity of fluorescent dye binding to double-stranded DNA (dsDNA). Qubit is generally considered useful for checking DNA quality before next-generation sequencing because it measures intact dsDNA.

What is RNA IQ?

The RNA IQ# is a value from 1 to 10, similar to other RNA quality scores, where a small number indicates that the sample is comprised of mainly small RNA and a larger number indicates that the sample consists of mainly large RNA or RNA with tertiary structure.

How do you quantify RNA?

The traditional method for assessing RNA concentration and purity is UV spectroscopy. The absorbance of a diluted RNA sample is measured at 260 and 280 nm. The nucleic acid concentration is calculated using the Beer-Lambert law, which predicts a linear change in absorbance with concentration (Figure 1).

Does qubit use PicoGreen?

The Quant-iT PicoGreen dsDNA assay is well-adapted to high-throughput use, typically in a 96-well plate. Qubit dsDNA assay kits are convenient for fewer samples, since the samples are read one at a time. The Qubit assays are available as a HS (high sensitivity) assay kit and a BR (broad range) assay kit.

Is qubit a fluorometer?

The Invitrogen Qubit 4 Fluorometer is the next generation of the popular benchtop fluorometer designed to accurately measure DNA, RNA, and protein quantity. The new Qubit 4 also easily measures RNA integrity and quality.

Can qubit measure ssDNA?

Qubit™ ssDNA Assay Kit. New—The Qubit™ 4 Fluorometer can now measure RNA quality in addition to providing accurate quantitation of dsDNA, ssDNA, RNA & protein.

What is qubit reagent?

Invitrogen Qubit assays utilize target-selective dyes that emit fluorescence when bound to DNA, RNA or protein, unlike traditional UV absorbance, which can overestimate sample concentrations due to contaminants in the sample such as salts, solvents, detergents, proteins, free nucleotides.

What is the difference between qubit and Nanodrop?

Devices using DNA binding dyes such as the qubit measure DNA concentrations directly, whereas the nanodrop quantifies anything that fluoresces at the approximate wavelength of DNA.

How does the qubit RNA BR assay kit work?

Description The Qubit RNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein or free nucleotides.

Is the Qubit 4 fluorometer selective for RNA VS DNA?

UV absorbance readings are not selective for RNA vs DNA. The Qubit 4 Fluorometer and all Qubit assays are optimized for use together. The Qubit 4 Fluorometer detects fluorescent dyes that bind specifically to the target of interest. This target specific enables precise, accurate measurements even for dilute samples requiring high sensitivity.

What kind of PCR is used for qubit?

All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers. 2. 500 µL thin-walled PCR tubes are required but not included. For Research Use Only. Not for use in diagnostic procedures. Qubit is specific for DNA or RNA. Store in refrigerator (2–8°C) and protect from light.

Which is the most accurate RNA quantitation kit?

The Qubit RNA HS (High Sensitivity) Assay Kits make RNA quantitation easy and accurate. The kits include concentrated assay reagent, dilution buffer, and prediluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume from 1–20 μL is acceptable), then read the concentration using the Qubit Fluorometer.