What is NeuN staining used for?

Monoclonal antibodies to the NeuN protein have been actively used in immunohistochemical studies of neuronal differentiation to assess the functional state of neurons in norm and pathology for more than 20 years. Currently, they are also used in the differential morphological diagnosis of cancer [8-10].

What is anti NeuN antibody?

Anti-NeuN antibodies are readily available from several suppliers. NeuN is a reported synonym of the human protein ‘RNA binding fox-1 homolog 3’, encoded by the gene RBFOX3. The full protein is reported to be 312 amino acid residues in length. The NeuN antigen is a documented neuronal marker.

What does NeuN bind to?

The Fox-3/NeuN antibody binds to the small cerebellum granule cell neurons, which form a prominent layer.

Does NeuN stain all neurons?

NeuN, an antibody derived from mice inoculated with purified neuronal nuclei, is specific to an unknown nuclear protein expressed by nearly all neurons and no glial cells. Unlike Nissl staining, this allows neurons to be visualized separately from glia.

Which is anti-Neun antibody detects level of Neun?

Anti-NeuN Antibody, clone A60 detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH (P), IP and WB. More>> Anti-NeuN Antibody, clone A60 detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH (P), IP and WB.

How to stain brain tissue with Neun ab104225?

ab104225 staining NeuN in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr – frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton-X and blocked with 5% serum for 1 hour at 26°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 12 hours at 4°C.

How is Neun antibody ab177487 used in tissue clearing?

Lane 3-4: 1 minute. Blocking and dilution buffer: 5% NFDM/TBST. NeuN antibody ab177487 was used with Tissue Clearing Kit ab243298 to penetrate, stain and clear a 1 mm coronal section of mouse brain.

Is the Neun antigen in the distal cytoplasm?

Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus.