Users' questions

What is used in biolistic or gene gun technique?

What is used in biolistic or gene gun technique?

The biolistic procedure, also known as the “gene gun”, involves firing small metal particles coated with the construct DNA into plant cells. The metal particles, usually gold or tungsten, are accelerated to high speed by the rapid release of high pressure helium in the gene gun into the target cells.

What is biolistic transformation?

Biolistic transformation enables the direct introduction of DNA or RNA into plant cells. In this method, a foreign DNA or RNA construct is coated onto gold or tungsten particles. The particles are released from a gene gun by high-pressure helium gas and directly penetrate the host cell wall [22].

Which is better Agrobacterium tumefaciensmediated transformation or biolistic gun?

Agrobacterium tumefaciensmediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient.

What is a gene construct in biolistic transformation?

Biolistic transformation involves the integration of a functional fragment of DNA—known as a DNA construct—into target cells. A gene construct is a DNA cassette containing all required regulatory elements for proper expression within the target organism.

How is the gene gun used in plant transformation?

The new plant that originated from a successfully transformed cell may have new traits that are heritable. The use of the gene gun may be contrasted with the use of Agrobacterium tumefaciens and its Ti plasmid to insert DNA into plant cells. See transformation for different methods of transformation in different species.

Which is the most versatile method of genetic modification?

Biolistics has proven to be a versatile method of genetic modification and it is generally preferred to engineer transformation-resistant crops, such as cereals. Notably, Bt maize is a product of biolistics.