What is Irma test?

This test is useful for detecting over 90 known and novel mutations in ABL kinase domain of Ph chromosome including T3151 and P-loop mutations responsible for resistance to Imatinib in CML patients.

What is T315I mutation?

Discussion. T315I mutation is a single base substitution act>att resulting in a threonine-to-isoleucine exchange at amino acid position 315 on BCR-ABL1 kinase domain.

What is BCR ABL mutation?

BCR-ABL is a mutation that is formed by the combination of two genes, known as BCR and ABL. It’s sometimes called a fusion gene. The BCR gene is normally on chromosome number 22. The ABL gene is normally on chromosome number 9. The BCR-ABL mutation happens when pieces of BCR and ABL genes break off and switch places.

When to test for BCR-ABL1 t3151 mutation?

BCR-ABL1 kinase domain mutation testing is recommended to guide choice of second-line TKI therapy. The BCR-ABL1 T3151 mutation imparts resistance to all currently approved TKIs. Content in tables below based on Cortes J, 2011; Hochhaus A, 2011; Hughes, 2009; Kantarjian H, 2007; Saglio G, 2012; Yeung, 2011.

Are there any patients with the T315I mutation?

A total of 38 (18%) patients harbored the T315I mutation in this period. According to the type of salvage therapy, patients were divided into subgroups of hematopoietic stem cell transplantation (HSCT) recipients (n = 9) and HSCT nonrecipients (n = 29).

Which is the mutation that imparts resistance to TKIs?

A 5- to 10-fold increase in the levels of BCR-ABL1 fusion transcripts by quantitative PCR suggests resistance. BCR-ABL1 kinase domain mutation testing is recommended to guide choice of second-line TKI therapy. The BCR-ABL1 T3151 mutation imparts resistance to all currently approved TKIs.

Are there any clinical trials with ABL1 T315I?

ABL1 T315I serves as an inclusion eligibility criterion in 7 clinical trials, of which 6 are open and 1 is closed. Of the trials that contain ABL1 T315I as an inclusion criterion, 2 are phase 1 (1 open), 2 are phase 1/phase 2 (2 open), 2 are phase 2 (2 open), and 1 is phase 3 (1 open).