Users' questions

Is cholesterol a fluorescent?

Is cholesterol a fluorescent?

Dansyl-cholesterol is another fluorescent cholesterol analog used in cellular studies [73, 74]. The Dansyl moiety was linked to carbon 6 of the steroid ring system, and recent fluorescence studies found that the Dansyl-group of this sterol is localized on average 1.56 nm from the bilayer center [75].

Do lipids fluoresce?

This technique is based on the observation that fluorescent lipids induce highly efficient and rapid membrane fusion between cellular plasma membranes and the lipid bilayers of their carrier particles containing neutral and positively charged lipids (Figure 1a,b). Liposomes of that kind are called fusogenic liposomes.

What does filipin stain?

Since filipin is highly fluorescent and binds specifically to cholesterol, it has found widespread use as a histochemical stain for cholesterol. This method of detecting cholesterol in cell membranes is used clinically in the study and diagnosis of Type C Niemann-Pick disease.

What is bodipy staining?

Description. With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY® 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids.

Which is the best fluorescent label for lipids?

Pyrenedecanoyl is of the most common fluorescent labels and is commonly incorporated as a head group in phospho- and glycerophospholipids. Pyrene labeled lipids are particularly useful for monitoring membrane fusion and phospholipid transfer processes due to their excimer-forming properties.

What are fluorescently labeled LDLs and acetylated LDLs?

Fluorescently labeled oxidized LDLs and acetylated LDLs. Alterations in lipid metabolism are known to be the root defect in atherosclerosis.

How are fluorescent conjugates used to study lipid metabolism?

Fluorescent conjugates of LDL (native and modified) can be employed to study these pathways using fluorescence microscopy, high-content analysis, and flow cytometry. Thermo Fisher Scientific offers unmodified LDLs and their fluorescent conjugates for studying LDL receptor–mediated endocytosis and other aspects of lipid metabolism.

Are there any fluorescent lipids made by Avanti?

Avanti offers a wide variety of fluorescent lipids to fulfill your research needs. There are several moieties that we use to install fluorescent properties in our lipids. As always, these lipids are made with the same quality standards as their non-fluorescent counterparts.