How to use Fuchs-Rosenthal counting chamber?

Directions for Use Fill the capillary of a 1:10 pipette to the graduation with the diluting fluid and then draw the spinal fluid to the graduation above the bulb. Shake the pipette and charge the chamber as with blood corpuscle counting technique. Allow several minutes for settling.

What is Fuchs-Rosenthal counting chamber?

The Fuchs-Rosenthal counting chamber is used to count white and red blood cells in CSF by observing them through a microscope on a calibrated grid, the exact dimensions of which are known. After it has been placed in a wet chamber, allow the liquid to settle before counting.

When to use the Fuchs-Rosenthal counting chamber?

The Fuchs-Rosenthal Counter is one piece construction ensuring durability and accuracy, featuring a Fuchs-Rosenthal ruling on a single plateau. TheFuchs-Rosenthal Counting Chamber is used for Cerebro-Spinal Fluid cell counts. (Cell-Depth 0.2mm)

How many cells are in a Fuchs Resenthal chamber?

It is generally recommended to count 16 one square millimeter areas preferably 8 in each chamber. Calculating Formula if 16 one square millimeter areas were counted: Note: For convenient calculation divide the cells counted by 3 instead of 3.2 and disregard the 10/9 dilution.

What is the cell depth of the Fuchs Rosenthal hemocytometer?

The cell depth in the Fuchs Rosenthal cell counting chamber is actually 0.2mm (see here) but the final value of 2×10-4 mL you’re using is the right one, and the final result as per your calculations looks good. I would recommend to check your hemocytometer counts: maybe count more than 5 squares]

How to calculate the formula for counting chambers?

Calculating Formula if 16 one square millimeter areas were counted: For convenient calculation divide the cells counted by 3 instead of 3.2 and disreguard the 10/9 dilution. The two fractions will compensate each other so that the remaining error will be negligible.