What is oligomycin poisoning?

What is oligomycin poisoning?

The Fo protein (the “o” in Fo refers to its sensitivity to oligomycin, a poison that blocks the flow of protons) extends through the inner mitochondrial membrane and serves as the proton channel between the intermembrane space and the matrix.

What does oligomycin do to the body?

Oligomycin A is an inhibitor of ATP synthase. In oxidative phosphorylation research, it is used to prevent state 3 (phosphorylating) respiration. Oligomycin A inhibits ATP synthase by blocking its proton channel (FO subunit), which is necessary for oxidative phosphorylation of ADP to ATP (energy production).

What does oligomycin do to NADH?

Oligomycin prevents the increase in mitochondrial respiration induced by ADP without inhibiting uncoupler-stimulated respiration. NADH remains high and NAD+ is too low for the citric acid cycle to operate.

Can oligomycin cause death?

Oligomycin, an inhibitor of the reversible mitochondrial ATP synthase (F1F0-adenosinetriphosphatase), caused dose-dependent cell killing with 0.1 microgram/ml being the minimum concentration causing the maximum cell killing. Fructose retarded cell death caused by CCCP but failed to prevent lethal cell injury.

What is the formula for the drug oligomycin?

More… Oligomycin A is an oligomycin with formula C45H74011. An inhibitor of mitochondrial F1FO ATP synthase that induces apoptosis in a variety of cell types and exhibits antifungal, antitumour, and nematicidal activities, but its clinical application has been limited by poor solubility in water and other biocompatible solvents.

How does oligomycin affect the electron transport chain?

The inhibition of ATP synthesis by oligomycin A will significantly reduce electron flow through the electron transport chain; however, electron flow is not stopped completely due to a process known as proton leak or mitochondrial uncoupling. This process is due to facilitated diffusion of protons into the mitochondrial matrix…

How are oligomycins used in the treatment of cancer?

Oligomycins have been used extensively to examine various mechanistic aspects of the ATP formation and energy requirements in tumor cell biology and apoptosis.

What kind of enzyme is oligomycin an inhibitor of?

Oligomycins are macrolides created by Streptomyces that can be poisonous to other organisms. They have use as antibiotics . Oligomycin A is an inhibitor of ATP synthase.