Where is Zo-1 found?

In the inner nuclear layer (INL) and the ganglion cell layer (GCL), ZO-1 is located at tight junctions between vascular endothelial cells (arrows).

What is Zonula occludens1?

Zonula occludens (ZO)-1 was the first tight junction protein to be cloned and has been implicated as an important scaffold protein. It contains multiple domains that bind a diverse set of junction proteins.

What does ZO1 stand for?

ZO-1

Which is the best definition of immunofluorescence?

immunofluorescence – (immunology) a technique that uses antibodies linked to a fluorescent dye in order to study antigens in a sample of tissue. technique – a practical method or art applied to some particular task. immunology – the branch of medical science that studies the body’s immune system.

How are fluorochrome antibodies prepared for immunofluorescence?

Preparation of fluorescence. To make fluorochrome-labeled antibodies, a fluorochrome must be conjugated (“tagged”) to the antibody. Likewise, an antigen can also be conjugated to the antibody with a fluorescent probe in a technique called fluorescent antigen technique.

How are occludin, ZO-1 and claudin-4 expressed in SCC cells?

In SCC, occludin, ZO-1, claudin-1 and claudin-4 were strongly expressed in tumour cells with keratinization such as cancer pearls. Claudin-1 was heterogeneously expressed in unkeratinized tumour cells, whereas expression of occludin, ZO-1 and claudin-4 was decreased or absent.

Why are tagged proteins used instead of immunofluorescence?

Use of such “tagged” proteins allows determination of their localization in live cells. Even though this seems to be an elegant alternative to immunofluorescence, the cells have to be transfected or transduced with the GFP-tag, and as a consequence they become at least S1 or above organisms that require stricter security standards in a laboratory.