What is the XhoI enzyme?

In molecular biology, XhoI is a type II restriction enzyme EC that recognise the double-stranded DNA sequence CTCGAG and cleaves after C-1. Type II restriction endonucleases (EC) are components of prokaryotic DNA restriction-modification mechanisms that protect the organism against invading foreign DNA.

What is the definition of one unit for XHOL?

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) fragments in 1 hour at 37°C in a total reaction volume of 50 µl.

Where does Xho1 cut?

Description. Thermo Scientific XhoI restriction enzyme recognizes C^TCGAG sites and cuts best at 37°C in R buffer.

What is CutSmart buffer?

Our CutSmart Buffer incorporates BSA to enable even more enzymes to cut in a single buffer (>200 enzymes). This allows for enhanced ease of use especially when doing double digests. In addition, it eliminates the extra tube of BSA and means one less thing to think about when setting up restriction enzyme digests.

What are the isoschizomers for FastDigest Xhoi?

Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI. Thermo Scientific FastDigest XhoI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

What kind of enzyme is Xhoi in lambda DNA?

Lambda DNA digested with XhoI, 0.7% agarose, 1 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Which is an isoschizomer of paer7i or Xhoi?

XhoI is an isoschizomer of PaeR7I. This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol. Impaired by CpG methylation.

How big is one unit of Xhoi enzyme?

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) fragments in 1 hour at 37°C in a total reaction volume of 50 µl. XhoI is an isoschizomer of PaeR7I.