What is the optimal specimen collection time to detect most viral infections?

For the detection of most viruses, specimens collected soon after the onset of clinical symptoms (preferably within the first 3–4 days) when viral shedding is greatest are preferred. Optimal specimens vary depending on the site or sites of disease. In general, tissues, aspirates, and body fluids are superior to swabs.

How do you store viral samples?

Hi, -80 should be fine to keep the virus. I think the trick is to place the small vials containing the virus into the -20degree freezer until it freezes and then transfer it into the -80. Keep the vials in a styrofoam holder so that the temperature shock to the virus is minimised.

What are the steps in specimen collection?

There are four steps involved in obtaining a good quality specimen for testing: (1) preparation of the patient, (2) collection of the specimen, (3) processing the specimen, and (4) storing and/or transporting the specimen.

How do you collect a viral culture?

Viral cultures: Specimens should be collected in the acute stage of the illness, kept moist, and refrigerated immediately. Stool specimens should not be placed into viral transport medium or frozen. Spinal fluid and throat washings must be kept cold and must not be frozen.

How are viral specimens used in the laboratory?

Viral Specimens or samples collection play vital role in laboratory diagnosis. Proper sample collection leads to proper diagnosis of disease. Sample should be collected aseptically. Appropriate sample from appropriate site and adequate amount should be collected.

When is the best time to collect a viral culture?

Viral cultures: Specimens should be collected in the acute stage of the illness, kept moist, and refrigerated immediately. Stool specimens should not be placed into viral transport medium or frozen.

How is virus culture collected in the UPMC?

VTM contains gelatin and antimicrobial agents in a buffered salt solution. The UPMC clinical virology lab requires specimens for virus culture be collected in the VTM M4 . Tubes containing 2-3 mL VTM are used for swab specimens, while those with 5-7 mL VTM are suitable for tissue samples.

What do you need to know about viral transport medium?

Viral transport medium (VTM) prevents specimen drying, maintains viral viability and retards the growth of microbial contaminants. VTM contains gelatin and antimicrobial agents in a buffered salt solution. The UPMC clinical virology lab requires specimens for virus culture be collected in the VTM ‘M4’.