Do exosomes carry RNA?

Exosomes contain proteins, microRNAs (miRNAs), and messenger RNAs (mRNAs) that can be transferred between cells. The discovery that exosomes contain RNA, and that this encapsulated RNA could potentially be transferred over distances in vivo, reinforced the importance of exosomes in cell-to-cell communication.

How do you identify an exosome?

Exosomes characterization. The characterization of exosomes can be summarized by its size, protein and lipid content. Usually they can be identified by detecting the morphology, particle size, and surface markers on the surface of exosomes.

What are in exosomes?

Exosomes are a type of extracellular vesicle that contain constituents (protein, DNA, and RNA) of the cells that secrete them. They are taken up by distant cells, where they can affect cell function and behavior.

Do exosomes contain DNA?

Exosomes are small extracellular vesicles that mediate biological and cellular functions including cell-to-cell communication (1). Recent studies have demonstrated the presence of genomic DNA (gDNA) and nuclear proteins within exosomes (7).

Who are the authors of the Exosome Diagnostics study?

Exosome Diagnostics and QIAGEN provided support in the form of salaries for authors DE, AS, CC, EB, RM, MS, MSH, JMS, EL, JS, MN, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

How is the exoeasy Maxi kit used to isolate exosomes?

The exoEasy Maxi Kit uses membrane affinity spin columns to efficiently isolate exosomes and other extracellular vesicles from serum, plasma, cell culture supernatant and other biological fluids. The maxi column format allows the use of large sample volumes, including up to 4 ml plasma or serum or up to 32 ml cell culture supernatant.

Is it possible to isolate exosomes from plasma?

Our exosome purification kits enable you to isolate exosomes and EV from all major biofluids, including exosome isolation from plasma. With our spin-column technology, you get comparable or better results than with ultracentrifugation – and you don’t need specialized equipment.

How to characterization of RNA from exosomes and other vesicles?

Citation: Enderle D, Spiel A, Coticchia CM, Berghoff E, Mueller R, Schlumpberger M, et al. (2015) Characterization of RNA from Exosomes and Other Extracellular Vesicles Isolated by a Novel Spin Column-Based Method. PLoS ONE 10 (8): e0136133. https://doi.org/10.1371/journal.pone.0136133