What is the purpose of MALDI-TOF?

Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory.

Can the following two peptides with the same amino acid composition be distinguished using MALDI-TOF mass spectrometry?

Can the following two peptides with the same amino acid composition be distinguished using MALDI-TOF mass spectrometry? A. No they cannot be distinguished because they have the same mass.

What is MALDI-TOF How does it work what can it tell us?

In the case of MALDI-TOF, the analyzer separates molecules based on the time it takes each of them to fly through the time-of-flight tube or “drift” region to the detector. The ionized sample molecules are accelerated by a high-voltage current and fly through the tube before striking the detector.

How are proteins mixed in MALDI TOF mass spectrometry?

In MALDI-TOF mass spectrometry, protein or peptide samples are mixed with a matrix compound (usually an aromatic organic acid that donates a proton to the analyte) and dried onto a metal sample plate.

How does MALDI TOF based protein fingerprinting work?

In the MALDI-TOF based protein fingerprinting method, a sample is digested with certain proteolytic enzyme (usually trypsin) and one MS spectrum is acquired which generates the massed of all peptides (or MH+), and these masses are used as a fingerprint to search proteins in a database.

How is MALDI-TOF-MS used in the laboratory?

Recently, the method was also successfully applied to DNA sequencing as well as screening for mutations. Thus, high-throughput genotyping of single nucleotide polymorphisms has the potential to become a routine method for both laboratory and clinical applications. Publication types

Which is the ion source in MALDI TOF mass analyzer?

In MALDI-TOF mass spectrometry, the ion source is matrix-assisted laser desorption/ionization (MALDI), and the mass analyzer is time-of-flight (TOF) analyzer. MALDI. MALDI is a soft ionization that involves a laser striking a matrix of small molecules to make the analyte molecules into the gas phase without fragmenting or decomposing them.